|
KMID : 0366219880230010177
|
|
Korean Journal of Hematology
1988 Volume.23 No. 1 p.177 ~ p.181
|
|
|
Preparation of cDNA Clone Bank from TPA-PHA Stimulated Human Lymphocyte by Recombinant DNA Technique
|
|
이수영/Soo Young Lee
김상호/이정용/유남진/박원상/김주성/Sang Ho Kim/Jung Yong Lee/Nam Jin Yoo/Won Sang Park/Choo Seung Kim
|
|
|
Abstract
|
|
|
In order to investigate the immunoregulatory and tumoricidal effects of Iymphokines
from human Iymphocytes stimulated with cancer promoter, TPA(12-o-tetradecanoyl
phorbol 13-acetate) and PHA-p, cDNA clone bank from the TPA and PHA-p stimulated
human Iymphocyte by recombinant DNA technique has been established. Messenger
RNAs purified from total cellular RNAs of the TPA and PHA-p stimulated human
Iymphocytes have been fractionated by sucrose density gradient centrifugation. 32
m-RNA fractions protein synthesis has been shown in 13 fractions by in vitro
translation.
The cDNAs have been synthesized on the oligo(dT) -tailed pBR322-SV40 primer by
the use of the corresponding mRNAs as templates, followed by annealing with the
oligo(dG) -tailed pBR322-SV40 linker and replacement of RNA strands by DNA.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|